9KYK
Crystal structure of human TRIP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.156, 64.298, 68.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.130 - 1.020 |
| R-factor | 0.1875 |
| Rwork | 0.187 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5156) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.060 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.076 | 0.416 |
| Rmeas | 0.088 | 0.483 |
| Rpim | 0.043 | 0.241 |
| Number of reflections | 72287 | 7222 |
| <I/σ(I)> | 9.3 | 5.4 |
| Completeness [%] | 98.5 | 99.2 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.955 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES pH7.5 and 20% (w/v) polyethylene glycol 8,000 |
