9KYJ

Crystal structure of mouse Endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1), Selenomethionine derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97623
Spacegroup name	P 21 21 2
Unit cell lengths	51.128, 96.102, 33.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.140 - 1.570
R-factor	0.1839
Rwork	0.182
R-free	0.21040
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.857
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL2Map
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.570	1.570
Rmerge	0.084	0.353
Rmeas	0.088	0.368
Rpim	0.025	0.103
Number of reflections	23707	2320
<I/σ(I)>	3.2	1.1
Completeness [%]	100.0	100
Redundancy	12.7	12.4
CC(1/2)	0.985	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400, 2.0 M Ammonium sulfate