9KY9
Crystal structure of E. coli tryptophanyl-tRNA synthetase complexed with 3-methylchuangxinmycin and tryptophanyl-5'-AMP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.761, 90.851, 74.753 |
| Unit cell angles | 90.00, 109.47, 90.00 |
Refinement procedure
| Resolution | 70.480 - 2.190 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8i1w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.994 |
| Data reduction software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.480 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.126 | 0.489 |
| Number of reflections | 40144 | 2936 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 280 | 0.2 M ammonium sulfate, 0.1 M MES, 20% PEG 8000 |
