9KVB
Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.560, 40.710, 63.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.420 - 2.770 |
| R-factor | 0.2616 |
| Rwork | 0.253 |
| R-free | 0.34200 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.948 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.420 | 2.869 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmeas | 0.165 | |
| Rpim | 0.083 | 0.511 |
| Number of reflections | 9675 | 411 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 95.2 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.2 M NH4CH3COO (PH8.5), 27% (w/v) PEG3350 |
