9KV9

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation E141G-Ca

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 2 21
Unit cell lengths	39.799, 63.612, 78.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.210 - 2.050
R-factor	0.241
Rwork	0.237
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.811
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.210	2.123
High resolution limit [Å]	2.050	2.050
Rmeas		0.481
Rpim	0.044	0.227
Number of reflections	13017	1267
<I/σ(I)>	8.1
Completeness [%]	99.7
Redundancy	5.4
CC(1/2)	0.995	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1M MMT (pH9.0), 25% (w/v) PEG 1500