9KV1

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation E141G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 2 21
Unit cell lengths	40.775, 63.882, 79.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.720 - 2.300
R-factor	0.2317
Rwork	0.225
R-free	0.29240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.828
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.720	2.379
High resolution limit [Å]	2.296	2.296
Rmeas	0.082	0.082
Rpim	0.034	0.034
Number of reflections	9663	874
<I/σ(I)>	5.8
Completeness [%]	99.0
Redundancy	6.1
CC(1/2)	0.994	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1M MMT (pH9.0), 25% (w/v) PEG 1500