9KUR

Crystal structure of mAb nCoV400Fab with SARS-CoV-2 N-CTD Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	48.408, 74.222, 96.510
Unit cell angles	106.63, 103.63, 96.07

Refinement procedure

Resolution	24.980 - 2.060
R-factor	0.2016
Rwork	0.199
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7de1 6tcn
RMSD bond length	0.002
RMSD bond angle	0.600
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.800	2.100
High resolution limit [Å]	2.060	2.060
Rmerge	0.101	0.576
Number of reflections	74510	11057
<I/σ(I)>	14.5	1.6
Completeness [%]	98.0
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M sodium formate, 16% PEG3350