9KUO
Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation Q136P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.505, 86.270, 126.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.790 - 3.130 |
| R-factor | 0.2139 |
| Rwork | 0.207 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.790 | 3.246 |
| High resolution limit [Å] | 3.130 | 3.134 |
| Rmeas | 0.131 | |
| Rpim | 0.342 | |
| Number of reflections | 7515 | 566 |
| <I/σ(I)> | 1.28 | |
| Completeness [%] | 91.1 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.998 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1M MMT (pH8.0), 25% (w/v) PEG 1500 |
