9KUI

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation F142L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 2 21
Unit cell lengths	41.623, 62.945, 82.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.720 - 2.680
R-factor	0.2097
Rwork	0.202
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.720	2.775
High resolution limit [Å]	2.679	2.679
Rmerge	0.131
Number of reflections	6145	439
<I/σ(I)>	2.49
Completeness [%]	94.5
Redundancy	4.9
CC(1/2)		0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1M Tris (pH8.5), 0.2 M Trimethylamine N-oxide dihydrate, 25% (w/v) PEG 3350