9KTQ

Crystal structure of the pathogen-secreted apoplastic GH12 xyloglucan-specific endoglucanase XEG1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	273
Detector technology	PIXEL
Collection date	2024-11-21
Detector	STFC Large Pixel Detector
Wavelength(s)	0.97861
Spacegroup name	I 2 3
Unit cell lengths	140.283, 140.283, 140.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.600 - 2.070
R-factor	0.1919
Rwork	0.190
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7w3v
RMSD bond length	0.005
RMSD bond angle	0.678
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	2.145
High resolution limit [Å]	2.070	2.071
Rmerge		0.579
Number of reflections	28081	2759
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	5% v/v TacsimateTM pH 7.0, 0.1 M HEPES pH 7.0, 10% w/v Polyethylene glycol monomethyl ether 5,000