9KSV
Complex structure of PcApiGT/UDP/Apigenin 7-O-glucoside
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.160, 75.160, 162.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.550 - 2.480 |
| R-factor | 0.2109 |
| Rwork | 0.205 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (Phenix1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.620 | 2.540 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 19600 | 1440 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19.1 | |
| CC(1/2) | 0.992 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 25% w/v polyethylene glycol 3,350, 0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5 |
