9KRW
E. coli MaeB apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 347.708, 200.289, 201.823 |
| Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
| Resolution | 49.470 - 3.850 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6znj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.536 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 3.920 |
| High resolution limit [Å] | 3.850 | 3.850 |
| Rmerge | 0.140 | 1.315 |
| Rmeas | 0.165 | 1.541 |
| Rpim | 0.087 | 0.798 |
| Number of reflections | 127049 | 6295 |
| <I/σ(I)> | 9.1 | 1.4 |
| Completeness [%] | 99.1 | 99.8 |
| Redundancy | 3.5 | 3.7 |
| CC(1/2) | 0.994 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M Potassium chloride, 20% w/v PEG 3350 |
