9KRS

Crystal structure of Horse spleen L-ferritin mutant (Fr-E53F/E56F/E57F/R59A/E60F/E63F)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-06-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	181.192, 181.192, 181.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.490 - 1.530
R-factor	0.1858
Rwork	0.184
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.016
RMSD bond angle	1.719
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.490	1.560
High resolution limit [Å]	1.530	1.530
Rmerge	0.060	0.883
Rmeas	0.063	0.939
Rpim	0.017	0.311
Number of reflections	38887	1880
<I/σ(I)>	30.8	2.4
Completeness [%]	99.9	100
Redundancy	12.8	8.7
CC(1/2)	1.000	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate