9KQL

The crystal structure of MORC2_CC3 domain at 3.1 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	I 41
Unit cell lengths	130.253, 130.253, 40.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.560 - 3.100
R-factor	0.2008
Rwork	0.198
R-free	0.24800
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.627
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.100
High resolution limit [Å]	3.050	8.270	3.050
Rmerge	0.112	0.062	1.214
Rmeas	0.117	0.065	1.275
Rpim	0.033	0.019	0.388
Total number of observations	85583
Number of reflections	6774	368	333
<I/σ(I)>	8.7
Completeness [%]	100.0	99.7	100
Redundancy	12.6	11.6	10.5
CC(1/2)	0.999	0.997	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1M Citric acid(pH 3.5) 25% w/v PEG 3350