9KQ9
The structure of the YcfA-GTP from Erwinia amylovora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 68.174, 134.366, 233.837 |
| Unit cell angles | 90.00, 98.35, 90.00 |
Refinement procedure
| Resolution | 47.510 - 2.590 |
| R-factor | 0.21331 |
| Rwork | 0.211 |
| R-free | 0.25746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.383 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.510 | 2.661 |
| High resolution limit [Å] | 2.590 | 2.594 |
| Number of reflections | 128775 | 12739 |
| <I/σ(I)> | 9.06 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.993 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M sodium malonate pH 7.0 and 20% PEG3350 |
