9KPX
UDP-glycosyltransferase TsUGT1 with UDP and 3-Glc-30-S-Glc-GA
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.869, 89.214, 113.774 |
| Unit cell angles | 94.36, 97.76, 104.72 |
Refinement procedure
| Resolution | 28.240 - 3.300 |
| R-factor | 0.158 |
| Rwork | 0.155 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.783 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15.1_3469: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.138 | 4.099 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.139 | 0.213 |
| Number of reflections | 13820 | 7099 |
| <I/σ(I)> | 5.2 | 1.1 |
| Completeness [%] | 86.2 | 69.8 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.978 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 20mM Tris, 100mM NaCl, 5 mM DTT |
