9KPQ
A ThDP-dependent enzyme belonging to the 1-deoxy-D-xylulose-5-phosphate synthase (DXPS)-like subfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97954 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.654, 71.787, 118.447 |
| Unit cell angles | 90.00, 97.05, 90.00 |
Refinement procedure
| Resolution | 48.660 - 2.420 |
| R-factor | 0.1955 |
| Rwork | 0.192 |
| R-free | 0.25664 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.781 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.102 | 0.426 |
| Number of reflections | 46470 | 4553 |
| <I/σ(I)> | 9.2 | 3.45 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Sodium malonate pH 6.0, 20% w/v Polyethylene glycol 3,350 |
