9KP4

Crystal structure of human CASTOR1 in apo form

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	83.768, 168.780, 98.761
Unit cell angles	90.00, 104.43, 90.00

Refinement procedure

Resolution	48.490 - 3.080
R-factor	0.2266
Rwork	0.225
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gs9
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	3.160
High resolution limit [Å]	3.070	3.070
Rmerge	0.112	0.730
Number of reflections	47329	3323
<I/σ(I)>	10.9	2
Completeness [%]	97.9	62.3
Redundancy	3.9	3.9
CC(1/2)	0.997	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Barium chloride, 30% v/v ethanol, 0.2 M NH4Ac, 0.1 M Tris pH 8.5, and 25% PEG 3350.