9KP2

Crystal structure of Horse spleen L-ferritin mutant (E56F/R59F) with Nile Red

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-04-26
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	F 4 3 2
Unit cell lengths	180.915, 180.915, 180.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.970 - 1.500
R-factor	0.1787
Rwork	0.178
R-free	0.19030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.016
RMSD bond angle	1.651
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.970	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.055	0.503
Rmeas	0.058	0.545
Rpim	0.017	0.207
Number of reflections	41026	1995
<I/σ(I)>	28.2	3.6
Completeness [%]	99.9	100
Redundancy	10.2	6.8
CC(1/2)	1.000	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Ammonium sulfate, Cadmium sulfate