9KOO

Ligand binding domain of Pseudomonas Aeruginosa PAO1 chemoreceptor PctA in complex with MHF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	82
Detector technology	CCD
Collection date	2023-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	70.972, 76.166, 117.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.240 - 2.700
Rwork	0.207
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t65
RMSD bond length	0.010
RMSD bond angle	1.221
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.240	2.700
High resolution limit [Å]	2.700	2.700
Number of reflections	18133	25707
<I/σ(I)>	11.16
Completeness [%]	99.3
Redundancy	7
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	2.0 M ammonium sulfate