9KO8

Crystal structure of Hook3(553-624) bound to KIF1C(714-809)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	54.562, 166.946, 39.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.671 - 3.000
R-factor	0.2465
Rwork	0.241
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9kns
RMSD bond length	0.010
RMSD bond angle	1.201
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	9356	371
<I/σ(I)>	10.3
Completeness [%]	93.6
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium citrate/citric acid (pH 5.5), 20% (w/v) polyethylene glycol 4000, and 20% (v/v) isopropanol