9KN1

Crystal structure of SARS-CoV-2 nucleocapsid phosphoprotein N-terminal domain(N-NTD) in complex with UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	58.753, 92.659, 97.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 2.120
R-factor	0.2275
Rwork	0.224
R-free	0.29280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.881
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	2.180
High resolution limit [Å]	2.120	2.120
Rmerge	0.155
Number of reflections	30878	2480
<I/σ(I)>	7.7
Completeness [%]	99.9	99.8
Redundancy	5.5
CC(1/2)	0.995	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	30% PEG3350, 100 mM Tris-HCl pH8.0, 5 mM UMP