9KLS

Crystal structure of a Streptococcal protein G B1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	67.256, 67.256, 86.686
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.420 - 2.510
R-factor	0.2466
Rwork	0.244
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.017
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.600
High resolution limit [Å]	2.510	2.510
Rmerge	0.056	0.600
Number of reflections	8112	787
<I/σ(I)>	33.3
Completeness [%]	100.0
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293.15	Protein concentration: 15.3 mg/mL Precipitant: 7.5% PEG 6000 (HAMPTON) Salt: 2.0 M sodium chloride