9KKR
Crystal structure of Horse spleen L-ferritin mutant (E53F/E56F/E57F/R59F/E60F/E63F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.430, 181.430, 181.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 1.500 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.649 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.070 | 0.771 |
| Rmeas | 0.073 | 0.824 |
| Rpim | 0.019 | 0.284 |
| Number of reflections | 41371 | 1999 |
| <I/σ(I)> | 24.5 | 2.3 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 12.4 | 8 |
| CC(1/2) | 0.999 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium sulfate, Cadmium sulfate |
