9KKP
Crystal structure of Horse spleen L-ferritin mutant (E53F/E56F/E57F/R59F/E60F/E63F) with Nile Red
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-01-19 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.511, 181.511, 181.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.799 - 1.600 |
| Rwork | 0.164 |
| R-free | 0.18580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.953 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.800 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.513 |
| Rmeas | 0.057 | 0.559 |
| Rpim | 0.017 | 0.217 |
| Number of reflections | 34271 | 1662 |
| <I/σ(I)> | 29.8 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 | 6.3 |
| CC(1/2) | 1.000 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium sulfate, Cadmium sulfate |
