9KKH

High resolution structure of Ferredoxin-NADP+ reductase from maize root - Reduced form, low X-ray dose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	P 32 2 1
Unit cell lengths	59.080, 59.080, 186.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.880 - 1.100
R-factor	0.1434
Rwork	0.143
R-free	0.17150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h59
RMSD bond length	0.005
RMSD bond angle	0.952
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.880	1.120
High resolution limit [Å]	1.100	1.100
Number of reflections	154328	7518
<I/σ(I)>	9.1	3.6
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	0.992	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293.15	PEG2000