9KKG
High resolution structure of Ferredoxin-NADP+ reductase from maize root - Oxidized form, low X-ray dose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.290, 59.290, 187.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.020 - 1.150 |
| R-factor | 0.1428 |
| Rwork | 0.142 |
| R-free | 0.15850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h59 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.948 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.020 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 136343 | 6686 |
| <I/σ(I)> | 10.1 | 3.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.995 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293.15 | PEG2000 |
