9KKC

Neutron structure of Ferredoxin-NADP+ reductase from maize root -Oxidized form

Experimental procedure

Experimental method	LAUE
Source type	SPALLATION SOURCE
Source details	J-PARC MLF BEAMLINE BL-03
Synchrotron site	JPARC MLF
Beamline	BL-03
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2022-03-02
Detector	iBIX
Wavelength(s)	2.05-5.38
Spacegroup name	P 32 2 1
Unit cell lengths	59.290, 59.290, 187.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.415 - 1.800
Rwork	0.190
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h59
RMSD bond length	0.008
RMSD bond angle	1.678
Data reduction software	STARGazer
Data scaling software	STARGazer
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.415	1.860
High resolution limit [Å]	1.800	1.800
Rpim	0.155	0.718
Number of reflections	36250	3539
<I/σ(I)>	6.34
Completeness [%]	99.5
Redundancy	9.0226
CC(1/2)	0.968	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293.15	PEG2000