9KHU

O-glycosyltransferase from Schisandra chinensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-01
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	54.366, 83.422, 93.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.800 - 2.200
R-factor	0.1897
Rwork	0.184
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.912
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.800	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.136	0.247
Number of reflections	21989	1996
<I/σ(I)>	7.6	3.9
Completeness [%]	99.4
Redundancy	5.8
CC(1/2)	0.983	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	25% PEG 3350, 0.1 M Tris