9KHB
Crystal structure of wild-type human fibrinogen gamma chain C-terminal domain (gamma-nodule)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2023-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.797, 66.656, 46.564 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 44.940 - 1.030 |
| Rwork | 0.119 |
| R-free | 0.13880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.940 | 1.050 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.049 | 0.714 |
| Rmeas | 0.054 | 0.776 |
| Rpim | 0.021 | 0.298 |
| Number of reflections | 103954 | 4920 |
| <I/σ(I)> | 17.4 | 2.2 |
| Completeness [%] | 97.4 | 93.3 |
| Redundancy | 6.6 | 6.6 |
| CC(1/2) | 0.999 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Reservoir solution (22% PEG 8000, 150 mM 2-Morpholinoethanesulfonic acid, pH 6.0, 70 mM calcium choride) Protein solution (20 mM 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, pH7.4, 120 mM sodium choride, 70 mM calcium choride) |
