9KHA

Crystal structure of maltodextrin-binding protein SPs0871 from Streptococcus pyogenes at 2.20 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	118.189, 118.189, 353.944
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.300 - 2.200
Rwork	0.185
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.462
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.084	0.352
Rpim	0.020	0.098
Number of reflections	47995	6775
<I/σ(I)>	21.9	6.8
Completeness [%]	98.4	89.4
Redundancy	17.3	13.4
CC(1/2)	0.999	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.13	Ammonium citrate tribasic 1.8 M