9KH2
Crystal structure of the first three zinc finger domains of ZBTB20 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.676, 147.788, 143.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.770 - 2.590 |
| R-factor | 0.1966 |
| Rwork | 0.194 |
| R-free | 0.24240 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.21_5207+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.083 | 0.991 |
| Number of reflections | 15677 | 1516 |
| <I/σ(I)> | 19.7 | 1.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.993 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.12 M Monosaccharides, 50 % v/v Morpheus Precipitant Mix 2, 0.1 M Buffer System 3, pH=8.5 |
