9KFM

Structure of WDR5 in complex with WIN motif containing EMBOW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9618
Spacegroup name	P 1
Unit cell lengths	47.194, 61.565, 64.761
Unit cell angles	68.91, 88.94, 67.63

Refinement procedure

Resolution	32.960 - 1.800
R-factor	0.1763
Rwork	0.175
R-free	0.20110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.121
Data reduction software	autoPX
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.108	0.624
Number of reflections	56477	4086
<I/σ(I)>	13.13
Completeness [%]	97.8
Redundancy	3.48
CC(1/2)	0.994	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium cacodylate, pH 5.5, 25% (w/v) PEG 4000