Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.900, 68.900, 79.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.450 - 1.590 |
| R-factor | 0.2241 |
| Rwork | 0.223 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.660 | 1.630 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.091 | 2.110 |
| Rmeas | 0.093 | 2.166 |
| Rpim | 0.022 | 0.487 |
| Total number of observations | 534416 | 42450 |
| Number of reflections | 29263 | 2156 |
| <I/σ(I)> | 21.4 | 1.6 |
| Completeness [%] | 98.2 | |
| Redundancy | 18.3 | 19.7 |
| CC(1/2) | 1.000 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277 | 2.6M AMMONIUM SULPHATE, 0.1M HEPES BUFFER PH7.5, 1% PEG 400 |
