9KFE

Truncated Fzo1 with modified LD, GTP-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 65 2 2
Unit cell lengths	77.133, 77.133, 284.664
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.220 - 2.810
R-factor	0.2237
Rwork	0.219
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	2.970
High resolution limit [Å]	2.810	2.810
Rmerge	0.128	0.196
Rmeas	0.154	0.236
Rpim	0.083	0.128
Number of reflections	12840	1775
<I/σ(I)>	9.8	6.5
Completeness [%]	98.6
Redundancy	5
CC(1/2)	0.991	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	150mM Cesium chloride, 17% PEG3350