9KF9
Crystal structure of ferritin mutant(E53H/E56H/E57H/R59H/E60H/E63H)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2024-04-08 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.543 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.639, 181.639, 181.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.191 - 1.500 |
| Rwork | 0.207 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.830 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.230 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 0.623 |
| Rmeas | 0.092 | 0.698 |
| Rpim | 0.033 | 0.310 |
| Number of reflections | 41233 | 2016 |
| <I/σ(I)> | 17 | 2.2 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 7.2 | 4.9 |
| CC(1/2) | 0.997 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Precipitant: (NH4)2SO4 (0.5-1.0M), CdSO4(12-20mM) Protein: 15-20mg/ml Crystallization drop: 1:1 |
