9KEA

Crystal structure of LnzB (Streptomyces spp. )

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-15
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	107.621, 162.475, 59.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.315 - 2.300
R-factor	0.207769246878
Rwork	0.206
R-free	0.24153
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.270
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.240	2.260
High resolution limit [Å]	2.140	2.140
Number of reflections	52784	52784
<I/σ(I)>	12.9
Completeness [%]	91.7
Redundancy	10.2
CC(1/2)	0.998	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.4 M Sodium phosphate monobasic, 1.6 M Potassium phosphate dibasic, 0.2 M NaCl and 0.1 M Imidazole, pH8.0