9KD5
Structure of WDR5 in complex with WIN motif containing Kif2A S121G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.612, 47.020, 103.488 |
| Unit cell angles | 90.00, 107.64, 90.00 |
Refinement procedure
| Resolution | 49.310 - 1.800 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.121 | 0.458 |
| Rmeas | 0.132 | 0.501 |
| Rpim | 0.052 | 0.200 |
| Number of reflections | 54747 | 11518 |
| <I/σ(I)> | 11.3 | 3.7 |
| Completeness [%] | 98.9 | |
| Redundancy | 6.4 | 6.2 |
| CC(1/2) | 0.993 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M imidazole malate, pH=5.5, 24% PEG 600 |
