9KBU
Crystal structure of an ankyrin protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-07-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953732 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 119.545, 119.545, 59.575 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.130 - 1.800 |
| R-factor | 0.1768 |
| Rwork | 0.176 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mj0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.123 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.773 | 1.829 |
| High resolution limit [Å] | 1.798 | 1.798 |
| Number of reflections | 45789 | 4525 |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20.9 | |
| CC(1/2) | 1.000 | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl pH 8.5, 2.5 M ammonium sulfate |
