9K9B

Apo form of Solanum tuberosum solanesyl diphosphate synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	C 1 2 1
Unit cell lengths	150.694, 134.593, 101.045
Unit cell angles	90.00, 98.69, 90.00

Refinement procedure

Resolution	44.725 - 2.189
R-factor	0.2031
Rwork	0.201
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.725	2.267
High resolution limit [Å]	2.189	2.189
Number of reflections	101617	9744
<I/σ(I)>	10.95
Completeness [%]	99.2
Redundancy	10.429
CC(1/2)	0.997	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium acetate, PEG 4000, MPD