9K8Z

Crystal structure of DNA/RNA duplex obtained using the counter diffusion method in space (K form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 61
Unit cell lengths	48.656, 48.656, 45.738
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.330 - 1.400
R-factor	0.1862
Rwork	0.185
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u6m
RMSD bond length	0.006
RMSD bond angle	1.037
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.330	24.330	1.440
High resolution limit [Å]	1.400	5.000	1.430
Rmerge	0.038	0.025	0.351
Rmeas	0.042	0.028	0.386
Number of reflections	12207	285	222
<I/σ(I)>	20.74
Completeness [%]	99.9
Redundancy	5.5
CC(1/2)	0.999	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION		293.15	potassium chloride, MPD, spermine, MES