9K8M
Hypoxanthine-guanine phosphoribosyltransferase from E. coli with HEPES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-08 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.217, 85.217, 168.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.940 - 2.080 |
| R-factor | 0.20509 |
| Rwork | 0.204 |
| R-free | 0.22692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.908 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.940 | 2.130 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.130 | 0.430 |
| Number of reflections | 40751 | 2877 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.7 | 96.49 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | ammonium sulfate |
