9K6M

Crystal structure of the Dictyostelium discoideum mitochondrial calcium uptake protein (DdMICU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	I 41 2 2
Unit cell lengths	103.937, 103.937, 297.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.109 - 2.502
Rwork	0.221
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	28688	3190
<I/σ(I)>	18.5	5.2
Completeness [%]	99.9
Redundancy	25.4
CC(1/2)	0.998	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M MIB buffer (Molecular Dimensions) pH 5.5, 20% (w/v) PEG 1500, 10 mM 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS), and 10 mM tris(2-carboxyethyl)phosphine (TCEP)