9K4F
SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class2
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.27 |
| Software | cryoSPARC,EPU |
| CTF correction | NONE |
