9K2T

Crystal structure of HLA-C*1202 in complex with IY10 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-26
Detector	RAYONIX MX-300
Wavelength(s)	0.900
Spacegroup name	C 2 2 21
Unit cell lengths	44.331, 116.168, 181.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.900 - 2.200
R-factor	0.2168
Rwork	0.215
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.992
Data reduction software	XDS (21-Sep-2017)
Data scaling software	Aimless (0.5.27)
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.091	0.874
Rmeas	0.098	0.940
Rpim	0.036	0.344
Number of reflections	24233	2050
<I/σ(I)>	16.6	2.1
Completeness [%]	99.9
Redundancy	7.4
CC(1/2)	0.999	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG