9K2R

Human T cell receptor (TRAV24*01/TRBV27*01) in complex with HLA-C*1202 and IY11 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98000
Spacegroup name	C 2 2 21
Unit cell lengths	207.827, 344.872, 134.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.500 - 2.980
R-factor	0.2085
Rwork	0.207
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	3.030
High resolution limit [Å]	2.980	2.980
Number of reflections	98231	4820
<I/σ(I)>	8.1
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.993	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M HEPES pH7.0, 1.6M ammonium sulfate, 0.2M sodium thiocyanate