9K1M

Crystal structure of xylanase inhibitor protein (OsXIP) from rice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	61.348, 68.743, 81.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.567 - 1.300
R-factor	0.1663
Rwork	0.166
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.339
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.600	1.370
High resolution limit [Å]	1.300	1.300
Number of reflections	161647	25158
<I/σ(I)>	15.46
Completeness [%]	99.1
Redundancy	6.6
CC(1/2)	0.999	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M BIS-TRIS pH 5.5, 25% PEG 3350