9K1K
Yeast Cytosine Deaminase mutant-M100H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-08-30 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 43 |
| Unit cell lengths | 79.077, 79.077, 55.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.958 - 1.439 |
| Rwork | 0.127 |
| R-free | 0.14420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.326 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.439 | 1.440 |
| Rpim | 0.025 | 0.122 |
| Number of reflections | 62709 | 6120 |
| <I/σ(I)> | 31.3 | |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M sodium cacodylate, 0.16M sodium acetate, 24% PEG 8000 |
