9JZF

Crystal structure of OsSPS3 complexed with zoledronate and isopentenyl diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	57.048, 90.718, 125.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.293 - 2.446
R-factor	0.2636
Rwork	0.261
R-free	0.31760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.474
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.293	2.534
High resolution limit [Å]	2.446	2.446
Number of reflections	24483	2390
<I/σ(I)>	10.61
Completeness [%]	98.7
Redundancy	10.244
CC(1/2)	0.989	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, Tris, Ammonium sulfate