Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.798, 68.798, 79.518 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.680 - 1.980 |
| R-factor | 0.2113 |
| Rwork | 0.208 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.092 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.680 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.101 | 1.008 |
| Rmeas | 0.105 | 1.045 |
| Rpim | 0.027 | 0.274 |
| Total number of observations | 202726 | 32672 |
| Number of reflections | 13766 | 2227 |
| <I/σ(I)> | 18.1 | 3.4 |
| Completeness [%] | 88.5 | |
| Redundancy | 14.7 | 14.7 |
| CC(1/2) | 0.999 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277 | 2.6M AMMONIUM SULPHATE, 0.1M HEPES BUFFER PH7.5, 1% PEG 400 |
